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In order to know some aspects of immune response of albino rats by Clonorchis sinensis infections, the histopathological changes of the liver and the chronological changes of large pyroninophilic cell(LPC) count in the spleen were observed. Rats of 5 experimental groups (A series), each group consisted of 5 rats, were infected with 50 metacercariae of C. sinensis, and they were kept for 3 days (Group Ⅰ), 7 days (Group Ⅱ), 28 days (Group Ⅲ), 56 days (Group Ⅳ) and 84 days (Group Ⅴ). The same number of experimental groups(B series), each group of rats inoculated with 50 adult worms of C. sinensis intraperitoneally, were also kept as same as the above A series. Rats of each experimental groups were sacrificed immediately after the respective infection period, and then microscopic specimens of the liver and spleen were prepared by staining the paraffin sections of removed tissues with hematoxylin-eosin stain and methyl green-pyronin stain. Those materials from the experimental groups were examined in comparison with the materials obtained from non-infected control groups. The results obtained were as follows: 1. The counts of LPC in the periarterial lymphatic sheath of spleen were rapidly increased and reached at the maximum on the 3rd day (Group Ⅰ) in both A and B series of rats. Thereafter, the LPC counts were gradually decreased. 2. LPC counts of A series of experimental group were generally higher than those of B series throughout the whole infection period. 3. Histopathologically the liver failed to show any parasitic worm inside the intrahepatic biliary system in the rats of B series. However, various pathological changes of the liver, which seemed to be induced by the toxic metabolites of C. sinensis inhabiting in the abdominal cavity, were observed in B series of rats.
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